Thesis on candida albicans

The purpose of this study was to assess the effect of a chitin derivative CSE on the adherence of Candida albicans to acrylic. Fungal adherence to acrylic dentures is considered an essential step in the development of denture stomatitis.

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Adherence of C. CSE was prepared from commercial chitin crab shell and from chitin isolated from C. The effect of both CSE types on the adherence of C. Both CSE types exerted a significant inhibitory effect when tested in the two experimental systems.


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Transcriptional and post-transcriptional gene silencing by RNA interference in Candida albicans

Inhibition of adherence of Candida albicans to acrylic by a chitin derivative. Authors Authors and affiliations E. Segal I.

The 5 Best Natural Remedies for Candida Albicans

Kremer D. This is a preview of subscription content, log in to check access. Candida biofilms have become an increasingly important clinical problem. The widespread use of antibiotics, frequent use of indwelling medical devices, and a trend towards increased patient immuno-suppression has resulted in a creation of opportunity for clinically important yeasts to form biofilms.

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Whilst there is growing evidence of the importance of Candida biofilms in clinical medicine, not all clinical isolates are able to form biofilms. There is therefore a fundamental gap in understanding exactly what drives biofilm formation and its clinical implications. These structures have become increasingly recognised as a significant clinical problem.

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One of the major reasons behind this is the impact that these have upon treatment, as antifungal therapy often fails and surgical intervention is required. This places a large financial burden on health care providers. Therefore, the discovery of alternative antifungal agents to be used in the treatment of fungal biofilms is in great demand for the management of these infections.

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  • A panel of Candida albicans bloodstream isolates were assessed for their biofilm forming ability by using the crystal violet assay and measuring cellular surface hydrophobicity. Scanning electron microscopy was used to visualise differences in the clinical biofilms. The impact of amphotericin B AMB treatment was determined next by broth microdilution method to assess differences in susceptibility profiles of the clinical isolates.

    • Inhibition of adherence of Candida albicans to acrylic by a chitin derivative | SpringerLink.
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    • The virulence of these clinical isolates was evaluated in vivo using a Galleria mellonella model and transcriptional analysis used to assess the expression of various genes associated with C. Extracellular DNA eDNA in clinical biofilms was quantified using a microplate fluorescence assay and chitinase activity measured using a biochemical assay. Moreover, the potential of a novel antimicrobial agent Carbohydrate-derived fulvic acid CHD-FA was assessed against a panel of fungal and bacterial species.

      The mechanism of action of CHD-FA was determined using membrane assays include ATP release, and propidium iodide fluorescence, with various inhibitors used to determine whether CHD-FA activity is affected by known resistance mechanisms. The results from this study have shown C.